Surgical treatment for obstructive hypertrophic cardiomyopathy: a five-year single-center experience of 421 cases / 中华外科杂志

Objectives: To examine the short-term and mid-term effects of surgical treatment of obstructive hypertrophic cardiomyopathy (HCM) in one center. Methods: The perioperative data and short-term follow-up outcomes of 421 patients with obstructive HCM who received surgical treatment at Department of Cardiac Surgery, Zhongshan Hospital, Fudan University from January 2017 to December 2021 were analyzed retrospectively. There were 207 males and 214 females, aged (56.5±11.7) years (range: 19 to 78 years). Preoperative New York Heart Association (NYHA) classification included 45 cases of class Ⅱ, 328 cases in class Ⅲ, and 48 cases in class Ⅳ. Fifty-eight patients were diagnosed with latent obstructive HCM and 257 patients had moderate or more mitral regurgitation with 56 patients suffering from intrinsic mitral valve diseases. All procedures were completed by a multidisciplinary team, including professional echocardiologists involving in preoperative planning for proper mitral valve management strategies and intraoperative monitoring. A total of 338 patients underwent septal myectomy alone, and 59 patients underwent mitral valve surgery along with myectomy. A single transaortic approach was used in 355 patients, and a right atrial-atrial septal/atrial sulcus approach was used in 51 other patients. Long-handled minimally invasive surgical instruments were used for the procedures. Student t test, Wilcoxon rank sum test, χ2 test or Fisher exact test were used to compare the data before and after surgery. Results: The aortic cross-clamping time of septal myectomy alone was (34.3±8.5) minutes (range: 21 to 94 minutes). Eighteen patients had intraoperative adverse events and underwent immediate reoperation, including residual obstruction (10 patients), left ventricular free wall rupture (4 patients), ventricular septal perforation (3 patients), and aortic valve perforation (1 patient). Four patients died during hospitalization, and 11 patients developed complete atrioventricular block requiring permanent pacemaker implantation. After discharge, 384 (92.1%) patients received a follow-up visit with a median duration of 9 months. All follow-up patients survived with significantly improved NYHA classifications: 216 patients in class Ⅰ and 168 patients in class Ⅱ (χ2=662.73, P<0.01 as compared to baseline). At 6 months after surgery, follow-up echocardiography showed that the thickness of the ventricular septum ((13.6±2.5) mm vs. (18.2±3.0) mm, t=23.51, P<0.01) and the peak left ventricular outflow tract gradient ((12.0±6.3) mmHg vs. (93.4±19.8) mmHg, 1 mmHg=0.133 kPa, t=78.29, P<0.01) were both significantly lower than baseline values. Conclusion: The construction of the surgical team (including echocardiography experts), proper mitral valve management strategies, identification and management of sub-mitral-valve abnormalities, and application of long-handled minimally invasive surgical instruments are important for the successful implementation of septal myectomy with satisfactory short-and medium-term outcomes.

Research ideas and method on screening active components of traditional Chinese medicine against hepatotoxicity with mitochondria as target / 中国中药杂志

Liver is the main place of drug metabolism. Mitochondria of hepatocytes are important targets of drug-induced liver injury. Mitochondrial autophagy could maintain the healthy operation of mitochondria in cells and the stable proliferation of cells. Therefore, the use of mitochondrial autophagy to remove damaged mitochondria is an important strategy of anti-drug-induced liver injury. Active ingredients that could enhance mitochondrial autophagy are contained in many traditional Chinese medicines, which could regulate the mitochondrial autophagy to alleviate relevant diseases. However, there are only a few reports on how to accurately and efficiently identify and evaluate such components targeting mitochondria from traditional Chinese medicine. Liquid chromatography-mass spectro-metry(LC-MS) combined with serum pharmacology in vivo can be used to accurately and efficiently find active ingredients of traditional Chinese medicine acting on mitochondrial targets. This paper reviewed the research ideas and methods of traditional Chinese medicine ingredients for increasing the hepatotoxicity of mitochondrial autophagy, in order to provide new ideas and methods for the study of active ingredients of traditional Chinese medicine targeting mitochondria.

Clinical features of children with coronavirus disease 2019 caused by Delta variant infection / 中国当代儿科杂志

OBJECTIVES@#To study the epidemiological and clinical features of children with coronavirus disease 2019 (COVID-19) caused by Delta variant infection and their differences from children with ordinary COVID-19 (non-Delta variant infection).@*METHODS@#Eleven children aged <14 years, who were diagnosed with COVID-19 caused by Delta variant infection from August to September 2021 were enrolled (variant group). Five children aged <14 years who were diagnosed with ordinary COVID-19 from February to March 2020 served as the control group. The epidemiological data, clinical features, and laboratory examination results were compared between the two groups.@*RESULTS@#There was no significant difference in the proportion of children with clinical symptoms between the two groups (@*CONCLUSIONS@#Delta variant is more infectious. COVID-19 caused by Delta variant infection may cause more serious myocardial damage than ordinary COVID-19 in children. In children infected with Delta variant, IgG antibody appears at almost the same time as IgM antibody.

Investigation on sea of marrow and the application of Naohu (GV 17) / 中国针灸

The theory on four seas is a kind of view to explain the longitudinal and horizontal distribution relationship of meridians and collaterals by ancient scholars. The authors were attempted to explore it by taking the theory on sea of marrow as example in terms of literature analysis. In (Chapter 33 of ), it is recorded that sea of marrow flows into two locations, but they are not the acupoint sites for treatment. Based on the comprehensive literature investigation, it is known that such two sites to which sea of marrow flows refer to a kind of boundaries so that the range of sea of marrow is determined, the function of it explored, and the controversy on "heart dominating mind" and "brain governing spirit" solved. It is defined that the function of sea of marrow is the macro-conception of (mind, spirit) that is modulated by vital movement information. Additionally, in association with meridian literature analysis, as well as acupoint textual research, it finally focuses on Naohu (GV 17) that is the critical point in the application of the theory on sea of marrow. In clinical application of such theory, the importance should be attached to the governor vessel, Naohu (GV 17) and their spatial system of bone.

Textual research of chu, huo, huo, and hua / 中国中药杂志

Origin of Chinese Characters (Shuowen Jiezi) records a large number of drug names, providing rich materials for the study of the history of pharmacy. But in the aftertime circulating process, errors were inevitable, making the ancient scholars disagree on the relationship between some drug names and their referents. This paper would give a textual research on medication names about chu, huo, huo, and hua. Relevant content was analyzed by using Shuowen Jiezi, Er Ya,Yu Pian and previous annotations of these exegesis books, as well as herbal literature. The relationship between the names and referents, as well as their lexical meanings were clarified by comparing textual content in specialized book about Chinese exegetics and previous herbal literature. Their medicinal efficacy was explained, and the value of herbalism in these exegesis books was explored.

Lycium barbarum polysaccharides attenuate oxidative stress injury of hu-man endothelium-like EA. hy926 cells induced by hydrogen peroxide / 中国病理生理杂志

AIM:To study the effect of Lycium barbarum polysaccharides (LBP) on oxidative stress injury of human endothelium-like EA. Hy926 cells induced by hydrogen peroxide (H2O2). METHODS:The EA. Hy926 cell model of oxidative stress injury was established by H2O2 treatment. The EA. Hy926 cells were divided into 5 groups:control group, damage (H2O2 at 50 mmol/L) group, LBP (100 mg/L) group, anti-damage groups (LBP at 50 mg/L, 100 mg/L or 200 mg/L+50 mol/L H2O2), and LY294002 (20 μmol/L) group. The effect of LBP at different concentrations on the cell viability of EA. Hy926 cells was measured by CCK-8 assay, and the optimum concentration of LBP was screened out. The apoptotic of EA. Hy926 cells was analyzed by flow cytometry. Acridine orange/ethidium bromide ( AO/EB) staining was used to observe the morphological characteristics of the apoptotic cells. The cell migration ability was detected by scratch method. The levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in the cell culture medium were examined. The protein levels of cleaved caspase-3, Bax, Bcl-2, endothelial NO synthase (eNOS), p-eNOS and p-Akt were determined by Western blot. RESULTS:LBP at concentration of 100 mg/L significantly attenuated the injury of EA. Hy926 cells induced by H2O2, as indicated by improved cell viability ( P <0.05 ) and decreased apoptosis ( P <0.05). Pretreatment with LBP elevated the levels of NO and VEGF (P<0.05), and promoted the migration ability of EA. Hy926 cells. LBP also increased the Bcl-2/Bax ratio, down-regulated the protein level of cleaved caspase-3, and up-regulated the protein levels of eNOS and p-eNOS. The protective effect of LBP were abolished by pretreatment of the EA. Hy926 cells with the inhibitor of PI3K (P<0.05). As a result, the protein level of p-Akt was down-regulated, and the level of NO was also significantly reduced. CONCLUSION:LBP has protective effect on H2O2-induced EA. Hy926 cells by attenuating apoptosis of the cells. The mechanism is closely related to the activation of PI3K/Akt/eNOS signaling pathway.

Risk factors analysis for low back pain in patients with multiple sclerosis / 中国骨伤

<p><b>OBJECTIVE</b>To investigate musculoskeletal disorders and risk factors of low back pain in multiple sclerosis (MS) patients.</p><p><b>METHODS</b>In this study, patients in our hospital with confirmed MS with an expanded disabilitystatus scale (EDSS) score between 4 to 7 were selected. Data of MS history, pain, musculoskeletal disorders, muscle strength and spasticity in lower limbs were collected.</p><p><b>RESULTS</b>Among 190 patients, there were 61 males and 129 females, with an average age of(54.9±9.2) years old. The mean disease duration was(19.3±9.9) years, and the median EDSS score was 6. Forty-two patients were relapsing-remitting type, 45 patients were primary progressive type, and 93 patients were secondary progressive type. The most common musculoskeletal disorders were described as follows:knee osteoarthritis (15 cases), claw toe (13 cases) and genu recurvatum (12 cases). Seventy-nine patients with prevalence low back pain was higher than in patients with a progressive type(secondary:=2.958,=0.007 9, primary(=2.629,=0.039 8) and in patients who had a visual dysfunction at EDSS score(=1.411,=0.012 4). The prevalence was reduced in male patients(=0.306,=0.001 4).</p><p><b>CONCLUSIONS</b>The progressive type of MS and visual dysfunction increased the risk of low back pain in these patients.</p>

Textual research of diao,ji,li,tiao,di / 中国中药杂志

Many medicine names were recorded in Shuowen Jiezi. The later scholars interpreted these names, but they had different standpoints on the relationship between the name and nature of some medicines. In this study, the medicine names of diao, ji, li, tiao, and di with greater different standpoints were verified by textual research. Shuowen Jiezi, Erya, Guangya and previous annotations of these exegesis books were combined with herbal literature to summarize and analyze the relevant content. The herbal content in exegesis books were compared with the records in herbal literature to hackle the relationship between name and nature of medicines, clarify the lexical meaning and illustrate the efficacy of the medicines. Meanwhile, the value of herbalism in these exegesis books was explored.

Assessment of the quality of clinical therapeutic research published in Chinese Journal of Pediatrics / 中华儿科杂志

<p><b>OBJECTIVE</b>To evaluate the methodological qualities of clinical therapeutic research papers published in Chinese Journal of Pediatrics and find out the weaknesses and defects in the literature of clinical therapeutic research in China.</p><p><b>METHODS</b>The clinical therapeutic research articles from Chinese Journal of Pediatrics, which were published in the years 1999, 2000, 2001, 2009, 2010 and 2011, were analyzed manually, after dividing into randomized controlled trials (RCT) and non-randomized controlled trials. The methodological quality of RCT articles were evaluated by Consolidated Standards for Reporting of Trials (CONSORT) and Jadad scale, while non-RCT reports were assessed by specific evaluation standard criteria and analytical table. And the group A (1999 - 2001) and group B (2009 - 2011) of the quality documents were compared and analyzed.</p><p><b>RESULTS</b>Seventeen RCTs were included. Based on the items in the CONSORT statement, the reporting quality of sample size determination, demographic baseline, random principle, blinding, lost to follow-up were all lower than 50%. Fourteen (82.4%) in the 17 RCTs mentioned 'eligibility criteria for participants', 11 (64.7%) RCTs mentioned 'details of the interventions', 15 (88.2%) RCTs mentioned 'adverse effects'. Compared with RCT reports in the year of 1999, 2000 and 2001, the methodological qualities of those in 2009, 2010 and 2011 were not improved. In the 17 RCTs, the total score achieved based on the Jadad scale, only 11.8% (2 out of 17) were high-quality trials (≥ 3 points). Analysis of the 28 non-RCTs, which were included and assessed by evaluation criteria and evaluation table, also showed some disadvantages on some key items.</p><p><b>CONCLUSIONS</b>The methodological quality of clinical therapeutic research reports should be consistent with the standards of international evaluation, pediatric clinicians should improve know ledge in clinical epidemiology, clinical trial methodology, and improve the quality of research reports.</p>

Effect of comprehensive control and prevention for chronic disease in demonstration plot of Chongqing / 中华预防医学杂志

<p><b>OBJECTIVE</b>To evaluate the effect of comprehensive control and prevention for chronic diseases in demonstration plot of Chongqing.</p><p><b>METHODS</b>Residents were enrolled through multi-stage stratified random sampling method from 17 districts or counties which had successfully established demonstration plots and 21 districts or counties which had not established demonstration plots (non-demonstration plot for short) yet on May, 2012. Questionnaire was designed to survey awareness of health knowledge, health behaviors and utilization of health supportive tools. The results were analyzed by SPSS 15.0 software.</p><p><b>RESULTS</b>We investigated 15 108 residents, 6156 of which were in demonstration plot and others (8951) were not. The findings revealed the percentage of the people who were aware the national action of health lifestyle in demonstration plot and in non-demonstration plot were 44.4% (2734/6157) and 40.2% (3598/8951), respectively, and the awareness of the hypertension risk of too much sodium were 72.4% (4458/6156) and 67.5% (6042/8951), respectively, and the awareness of the cardinal vascular disease (CVD) risk of obesity and overweight were 77.2% (4753/6157) and 69.6% (6230/8951), respectively. About the residents' health behaviors in demonstration plot and in non-demonstration plot, the utilization rates of salt restriction scoop or pot were 23.5% (1447/6157) and 17.9% (1602/8951), and the utilization rates of oil restriction pot were 16.7% (1028/6157) and 11.8% (1064/8951), respectively. Totally, 33 of the 37 indexes were shown higher in demonstration plot than that in non-demonstration plot (P < 0.05).</p><p><b>CONCLUSION</b>The chronic diseases comprehensive control and prevention in demonstration plot was more effective, and the remarkable improvement of health knowledge and behaviors level had been achieved in demonstration plot.</p>

Differentiation between glioma recurrence and radiation-induced brain injuries using perfusion-weighted magnetic resonance imaging / 中国医学科学院学报

<p><b>OBJECTIVE</b>To evaluate the role of perfusion weighted imaging (PWI) in the differentiation between recurrent glioma and radiation-induced brain injuries.</p><p><b>METHODS</b>Twenty-three patients with previously resected and irradiated glioma, presenting newly developed abnormal enhancement, were included in the study. The final diagnosis was determined either histologically or clinicoradiologically. PWI was obtained with a gradient echo echo-planar-imaging (GRE-EPI)technique. The normalized relative cerebral blood volume (rCBV) ratio [rCBV (abnormal enhancement)/rCBV (contralateral tissue)], relative cerebral blood flow (rCBF) ratio [rCBF (abnormal enhancement)/rCBF(contralateral tissue)], mean transit time(MTT) ratio [MTT (abnormal enhancement)/MTT(contralateral tissue)],time to peak(TTP)ratio[TTP(abnormal enhancement)/TTP(contralateral tissue)],and bolus arrive time(BAT)ratio[BAT(abnormal enhancement)/BAT(contralateral tissue)] were calculated. The regions of interest (ROIs) consisting of 20-40mm(2) were placed in the abnormal enhanced areas on postcontrast T1-weighted images.Ten ROIs measurements were performed in each lesion.T test was used to determine whether there was a difference in the rCBV/rCBF/rMTT/rTTP/rBAT ratios between recurrent glioma and irradiated injuries.Significance was set to a P value <0.05.</p><p><b>RESULTS</b>Thirteen of the 23 patients were proved recurrent glioma and 10 were proved radiation-induced brain injuries. The rCBV ratio (3.60±3.86 vs. 0.82 ± 0.74, P = 0.000)and rCBF ratio (2.88 ± 2.27 vs. 0.84 ± 0.80, P = 0.000) in glioma recurrence were markedly higher than those in radiation injuries. The areas under rCBV and rCBF ROC curve were both 0.8763. rMTT (P=0.204), rTTP (P=0.260), and rBAT (P=0.071) ratios showed no statistical difference between the two groups.</p><p><b>CONCLUSION</b>PWI is an effective technique in distinguishing glioma recurrence from radiation injuries,and the ratios of rCBV and rCBF ratio differential diagnosis of critical value to 1.3088 and 1.1235, respectively, can be used as a reliable clinical indicator.</p>

Application value of apparent diffusion coefficient in differentiation of brain radiation-injuries and glioma recurrence / 中国医学科学院学报

<p><b>OBJECTIVE</b>To evaluate application value of apparent diffusion coefficient (ADC) in differentiating brain radiation-injuries and glioma recurrence.</p><p><b>METHODS</b>Totally 23 patients [18 men and 5 women aged 32 to 67 years (mean: 47 years)] with previously resected and irradiated glioma were examined by using a 3.0T MR Scanner, including conventional and diffusion weighted image (DWI) sequences. All the cases were proved by pathology or clinical follow-up. Postprocessing of ADC maps was performed by using Functool software (AW 4.3, GE Healthcare), and the regions of interest (ROIs) were manually drawn on ADC maps in the area corresponding to the contrast-enhancing area on post-contrast axial T1-weighted images, avoiding the hemorrhage or necrosis. The ADC values were calculated automatically. ADC values were measured 10 times in order to reduce errors, and mean ADC value, maximum ADC value, and minimum ADC value were acquired.</p><p><b>RESULTS</b>Both recurrent glioma and irradiated necrosis tended to have hemorrhage, necrosis, and edema. The mean ADC value and maximum ADC value were lower in the recurrent tumor group than in the radiation-injuries group, but no statistical difference was achieved. However, the minimum ADC value was significantly lower in the recurrent tumor group than in the radiation-injuries group (P=0.016).</p><p><b>CONCLUSION</b>The minimum ADC value can be used as an useful tool in differentiating tumor recurrence from radiation-injuries.</p>

Analysis on the frequency of urinary iodine in a population-based intervention study / 中华流行病学杂志

Objective Through a two-year follow up program,this study was to analyze the urinary iodine frequency of a cohort in the intervention trial,concerning different doses of salt iodization,so as to explore the selection of appropriate concentration of salt iodization.Methods A multistage cluster sampling method was used to select three townships in two countries for community intervention with different doses [ ( 15 ± 5 ) mg/kg,(25 ± 5 ) mg/kg,( 35 ± 5 ) mg/kg ] of salt iodization.Results After intervention,the median of urinary iodine was reduced among the population.The urinary iodine frequencies of (15 ± 5) mg/kg and (25 ± 5) mg/kg among groups of children were mainly concentrated in 100-200 μg/L and 200-300 μg/L paragraphs in A county.While the 300 μg/Lparagraph had an overall decline in B county,the 100 μtg/L and 200 μg/L paragraph ratio increased but the trend seemed to be slow.The 100-300 μg/L paragraph of the four treatment groups took a larger proportion and kept smooth in a more ideal state.However,the control group still maintained at above 250 μg/L level.Conclusion The iodine supplementation should be gradually implemented in Chongqing.The doses of salt iodization should be reduced from the current (35 ± 15)mg/kg to (25 ± 5) mg/kg in the economically developed areas.At the same time,we need to continuously follow the changes of the condition.

Clinical observation on diabetic peripheral neuropathy treated with electroacupuncture and acupoint injection / 中国针灸

<p><b>OBJECTIVE</b>To compare the differences in the therapeutic effect on diabetic peripheral neuropathy between the combined therapy of electroacupuncture and acupoint injection and the simple acupoint injection.</p><p><b>METHODS</b>Under the satisfactory control of blood glucose, 60 cases of diabetic peripheral neuropathy were divided randomly into two groups, 30 cases in each one. In electroacupuncture plus acupoint injection group (group A), electroacupuncture and acupoint injection with Methylcobalamin were administered. Penetrating acupuncture was applied from Gongsun (SP 4) to Quanzhong (Extra) and from Yongquan (KI 1) to Taichong (LR 3) mainly. Acupoint injection was administered on Sanyinjiao (SP 6). In acupoint injection group (group B), only acupoint injection with Methylcobalamin was provided on Sanyinjiao (SP 6). After 2 sessions of treatment, the conduction velocity of ulnar nerve and tibial nerve was measured. The scores of Chinese medicine syndrome and diabetic peripheral neuropathy were recorded before and after treatment in two groups.</p><p><b>RESULTS</b>The effective rates were 90.0% (27/30) and 63.3% (19/30) in group A and group B respectively, presenting significant statistical difference (P < 0.05). After treatment, the motor nerve conduction velocity (MCV) and sensory nerve conduction velocity (SCV) of ulnar nerve and tibial nerve in group A were higher than those in group B (P < 0.05, P < 0.01). After treatment, the score of Chinese medicine syndrome in group A was lower than that in group B (14.36 +/- 1.88 vs 26.58 +/- 3.52, P < 0.01), the score of diabetic peripheral neuropathy in group A was lower than that in group B (12.86 +/- 4.28 vs 17.89 +/- 4.35, P < 0.01).</p><p><b>CONCLUSION</b>Electroacupuncture and acupoint injection with Methylcobalamin achieve a significant clinical efficacy on diabetic neuropathy and its efficacy is superior to that of simple acupoint injection with Methylcobalamin. This therapy can effectively increase nerve conduction velocity, control and relieve the symptoms of diabetic peripheral neuropathy.</p>

Surveillance on the health status regarding vector-borne disease among people living in the three gorges reservoir area (1997-2009) / 中华流行病学杂志

Objective To evaluate the impact on the health status of population in the area where the ‘Three Gorges Reservoir Water Storage Project' had been operated and to provide references for the development of related disease control strategies.Methods In the Three Gorges Reservoir Area,17 towns/streets in nine counties were chosen as surveillance points.Data on surveillance program would include infectious diseases,birth,death,biological agents etc.Methods related to time and spatial analysis and the comparison on pre- and post- water storage were used to analyze and describe the long-term changing trend of meteorological index,distribution of biological agent and diseases,and human health status.Relationships between number of infectious diseases and media biological density and meteorological parameters were also analyzed.Results When the water storage program came into being in 2003,the indoor (2.22％) and outdoor (2.76％) densities of rats were significantly lower than pre-water storage period (indoor 4.38％ vs.outdoor 4.43％) and the annual average density of mosquito (35.09 mosquitoes per room and per hour) was also lower than before (54.24 mosquitoes per room and per hour).The incidence rates of encephalitis B,malaria,leptospirosis,and hemorrhagic fever had reduces 22.88％,84.85％,95.03％ and 81.82％ than before.The incidence rates of malaria,leptospirosis,and hemorrhagic fever were all below 0.4/100 000 and the incidence of encephalitis B was below 2/100 000.The average infant mortality was 11.83‰.The annual infant mortalities,after adjusted by missing report numbers,were between 13.07‰ and 23.88‰ which were lower than the national annual average level.The thirteen year standard mortalities were 3.77‰-5.12‰,with the total rate lower than the national average level in the same years.Conclusion In 2003,the incidence rates of malaria,encephalitis B,leptospirosis,epidemic hemorrhagic fever kept going down in the surveillance sites in the Three Gorges Reservoir Area,and the overall population's health status was well enough to show that there was no negative impact on the health status of population living in the area after the water storage project was inplemented.

Molecular epidemiologic analysis on new emerged type 3 dengue virus in Guangzhou in 2009 / 中华流行病学杂志

Objective To analyze and trace the infection source the envelope(E) gene of the new emerged type 3 dengue virus in Guangzhou in 2009. Methods Sera were collected from patients infected with local dengue fever. Dengue virus was cultured and isolated by C6/36 cells. The whole length E gene was amplified from the positive specimen by RT-PCR, thereby sequenced and phylogenetic tree drawn by neighbor-joining method. Both data on epidemiologic and molecular studies were processed and analysed. Results 7 strains of type 3 dengue virus were isolated from samples of the 19 patients. E gene of these strains was amplified. The complete E genes of 7 strains belonged to 1479 nucleotides in length, encoding a polyprotein of 493 amino acids. Data from the phylogenetic analysis showed that 09/GZ/1081, 09/GZ/1483 and 09/GZ/10806 strains fell within the Southeast Asia/South Pacific group. 09/GZ/10616, 09/GZ/11144, 09/GZ/11194 while 09/GZ/13105 strains fell within the India group. Conclusion The type 3 dengue virus identified in Guangzhou area in 2009 was imported and could be devided into two genotypes.

Avian influenza virus infection in people occupied in poultry fields in Guangzhou city / 中华流行病学杂志

Objective To conduct serological investigation on H5N1/H9N2/H7N7 infection among people occupied in poultry fields. Methods Serum samples were collected from people working in live poultry and none-poultry retailing food markets, poultry wholesaling, large-scale poultry breading factories and in small-scale farms, wide birds breeding, swine slaughtering houses and from normal population. Antibodies of H5, H9 and H7 with hemagglutination inhibition and neutralization tests were tested and analyzed. Logistic regression and χ2 test were used. Results Among 2881 samples, 4 were positive to H5-Ab(0.14%), 146 were positive to H9-Ab (5.07%) and the prevalence of H9 among people from live poultry retailing (14.96%) was the highest. Prevalence rates of H9 were as follows: 8.90% in people working in the large-scale poultry breading factories, 6.69% in the live poultry wholesaling business, 3.75% in the wide birds breeding, 2.40% in the swine slaughtering, 2.21% in the non-poultry retailing, 1.77% in the rural poultry farmers and 2.30% in normal population. None was positive to H7-Ab among 1926 poultry workers. Conclusion The H5 prevalence among people was much lower than expected, but the H9 prevalence was higher. None of the populations tested was found positive to H7-Ab. There was a higher risk of AIV infection in live poultry retailing, wholesaling and large-scale breading businesses, with the risk of live poultry retailing the highest. The longer the service length was, the higher the risk existed.

Professor Sun Shen-tian's experience in treatment of mental diseases / 中国针灸

Professor SUN Shen-tian is an expert in acupuncture and moxibustion science of TCM and neurology, especially in the treatment of neurology difficult diseases by using the therapy of acupuncture and moxibustion and combination of TCM with western medicine, showing his ingenuity and obvious therapeutic effect. In treating mental diseases, professor SUN considers that the morbidity of mental diseases is mainly related to the malfunction of brain, therefore regulating mind is the key to treat this kind of disease, Baihui (GV 20), Yintang (EX-HN 3), emotional area and Zone 1 of SUN's abdominal acupoints are often selected. This present article introduces the principle of treatment based on syndrome differentiation and routine selecting acupoints, attached with four effective cases in treating mental diseases by professor SUN applying "the methods of regulating vitality and increasing intelligence".

Risk assessment of H5N1 human infection after an outbreak of avian influenza in water fowl / 中华预防医学杂志

<p><b>OBJECTIVE</b>To evaluate the risk of human infection after the outbreak of avian influenza H5N1 in animals, and probe the possibility for virus transmission.</p><p><b>METHODS</b>By means of field epidemiological study, molecular epidemiology, serology and emergency surveillance, persons who had ever closely contacted with sick or dead poultry were observed. While, the RT-PCR and gene sequencing method were used to detect H5 nucleic acid from environmental swabs from 4 epidemic spots, and hemagglutination inhibition assay was also used to detect H5 antibody.</p><p><b>RESULTS</b>Of 22 environmental swabs detected from 4 epidemic spots, one was positive for H5 nucleic acid, and the homogeneity was 95.9% as compared with H5N1 virus A/China/GD01/2006 (H5N1) found in Guangzhou in 2006 by gene sequence analysis. 62 environmental swabs from live poultry stalls of food markets near epidemic spot were detected negative. Six of 68 blood samples of contacts were positive for H9 antibody, and all were negative for H5 antibody. 68 throat swabs of contacts were detected negative for H5 nucleic acid. No close contact was found abnormal after 7 days medical observation. 337 influenza-like cases were reported in emergency surveillance, and no suspicious case was found.</p><p><b>CONCLUSION</b>The current outbreak of H5N1 avian influenza in water fowls has not yet caused further transmission, and human avian influenza case has not been observed. It indicates that the ability of H5N1 virus to transmit to human is not strong yet, and the risk of human infection for H5N1 is still low.</p>

MR image-guided anterior direct arthrography of the shoulder / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the method of MR guided anterior direct arthrogaphy of shoulder and its clinical applications.</p><p><b>METHODS</b>From 2008.4 to 2008.10, 40 patients with rotator-cuff tear (29 males and 11 females, ranging in age from 22 to 70 years, with an average of 35 years) underwent MR guided anterior direct arthrogaphy of shoulder, so as to diagnosis shoulder wound. The results were compared to that of arthroscopy.</p><p><b>RESULTS</b>Shoulder arthrography showed lesons in 36 patients, and failure in 2 patients, including contrast media extravasation in 1 patient, and gas entry to joint cavity in 1 patient. Two patients showed no rotator cuff injuries. Twenty-eight patients were confirmed by arthroscopy as rotator-cuff injured in different degree. Twenty-six patients were confirmed injuries by both arthroscopy and MR guided anterior direct arthrogaphy.</p><p><b>CONCLUSION</b>MR guided anterior direct arthrogaphy of shoulder as a minimally invasive method in displaying the rotator-cuff tear has higher accuracy and wide clinical application prospect.</p>